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<\/p>\nSuperox-D has been tested for its ability of being up taken and able to provide protection at intestinal level. A human intestine model of enterocyte-like cell Caco-2 was selected been widely used as a model of the intestinal epithelial barrier. It has been well documented that Caco-2 monolayers represent a reliable correlate for studies on the absorption of drugs and other compounds after oral intake in humans.<\/p>\n
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To assess the ability of being efficiently absorbed in the intestine, the Caco-2 cell model were incubated for 2 hours with media containing different concentration of Superox-D. The ability of Superox-D of being up taken was assessed measuring the Total Antioxidant Activity (TAA) of cell cytosol. The data reported in Figure show that Superox-D is able to effectively penetrate the cell membrane and to induce an increased antioxidant capacity in cell cytosol. This effect is due to the ability of Superox-D of being promptly up taken by the intestine proving the high bioavailability of the product.<\/p>\n
![\"\"](\"https:\/\/phnova.com\/wp-content\/uploads\/2018\/12\/In-vitro-studies2-300x158.png\")
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The protective effect of Superox-D was assessed by measuring the ability of some enterocyte-like cell Caco-2 model culture of better resisting to intense antioxidant stress. The cell lines were pre treated for 2 hours with media containing different concentration of Superox-D. After the incubation with Superox-D a fresh control medium was used in all samples and the effect of an oxidative stress caused by tert<\/i>-butyl hydroperoxide (t<\/i>-BOOH) was assessed with a fluorometric assessment. As shown in Figure the cell lines pre treated with Superox-D proved to be far more resistant to the radical stress induced during the assay with a dose dependent behavior. Superox-D is therefore able of keeping the intestine protected from the harmful radical species.<\/p>\n
![\"\"](\"https:\/\/phnova.com\/wp-content\/uploads\/2018\/12\/In-vitro-studies3-300x220.png\")
<\/p>\n","protected":false},"excerpt":{"rendered":"
Superox-D has been tested for its ability of being up taken and able to provide protection at intestinal level. A human intestine model of enterocyte-like cell Caco-2 was selected been widely used as a model of the intestinal epithelial barrier. It has been well documented that Caco-2 monolayers represent a reliable correlate for studies on […]<\/p>\n","protected":false},"author":1,"featured_media":155,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[13],"tags":[39],"class_list":["post-21","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sod","tag-superoxide-dismutases"],"_links":{"self":[{"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/posts\/21","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/comments?post=21"}],"version-history":[{"count":1,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/posts\/21\/revisions"}],"predecessor-version":[{"id":22,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/posts\/21\/revisions\/22"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/media\/155"}],"wp:attachment":[{"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/media?parent=21"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/categories?post=21"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/phnova.com\/en\/wp-json\/wp\/v2\/tags?post=21"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}